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Colorimetric Detection reagent Dilution table 1/2 Plate one Plate two Plates four Plates Colorimetric Detection Concentrate 140 L 260 L 500 L 1 mL Assay Buffer 1.26 mL 2.34 mL 4.5 mL 9 mL total Colorimetric reagent Volume 1.4 mL 2.6 mL 5 mL 10 mL reaction Mixture Prepare the Reaction Mixture by diluting one part each NADPH and Glutathione Reductase Concentrates 1:10 into eight parts Assay Buffer

The activation of PARP induces major neurotoxicity, as evidenced by the extreme protection against brain damage in PARP knockout mice subjected to transient MCA occlusion (up to 80% reduction of infarct volume) (349, 360)

10.1094/MPMI.2000.13.12.1380 21 CombetE.El MesmariA.PrestonT.CrozierA.McCollK